Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones

Abstract Background CRISPR/Cas9 editing systems are currently used to generate mutations in a particular gene to mimic a genetic disorder in vitro. Such “disease in a dish” models based on human pluripotent stem cells (hPSCs) offer the opportunity to have access to virtually all cell types of the hu...

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Detalhes bibliográficos

Principais autores:	Elie Frank, Michel Cailleret, Constantin Nelep, Pascal Fragner, Jérome Polentes, Elise Herardot, Lina El Kassar, Karine Giraud-Triboult, Christelle Monville, Karim Ben M’Barek
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2023-04-01
coleção:	Stem Cell Research & Therapy
Assuntos:	CRISPR/Cas9 Gene edition Human pluripotent stem cell Clonal isolation
Acesso em linha:	https://doi.org/10.1186/s13287-023-03327-2
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