Protocol to assess retinal metabolic flux of mice via stable isotope-resolved metabolomics

Summary: Here, we present a protocol for evaluating glucose metabolism in mouse retinas and retinal pigment epithelium (RPE)-choroid tissue by tracking the incorporation of 13C6 from U-13C6-glucose with gas chromatography-mass spectrometry (GC-MS). We describe steps for incubating tissues in Krebs-R...

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Principais autores:	Georgy Komissarov, Kriti Pandey, Nicholas D. Nolan, Thomas Winogrodzki, Daniel T. Hass, Aykut Demirkol, Brian M. Robbings, James B. Hurley, Stephen H. Tsang
格式:	Artigo
語言:	Inglês
出版:	Elsevier 2026-03-01
叢編:	STAR Protocols
主題:	Metabolism Metabolomics Mass spectrometry
在線閱讀:	http://www.sciencedirect.com/science/article/pii/S2666166725006586
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