Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells

Summary: Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed technique that combines single-molecule super-resolution microscopy and traditional FRAP microscopy. smFRAP enables researchers to measure the dynamics, spatial locations, and relative concentrations of...

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Hlavní autoři:	Mark Tingey, Yichen Li, Weidong Yang
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2021-06-01
Edice:	STAR Protocols
Témata:	Atomic Force Microscopy (AFM) Microbiology Molecular Biology Biotechnology and bioengineering
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S2666166721001970
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