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Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria

A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so the protocol could be useful in the study of vesicles...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Hauptverfasser: Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias, Elena Mercade
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-protocol LLC 2019-09-01
Schriftenreihe:Bio-Protocol
Online-Zugang:https://bio-protocol.org/en/bpdetail?id=3367&type=0
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