High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells
CRISPR/Cas9 technology has the potential to significantly enhance plant breeding. To determine the specificity and the mutagenic spectrum of SpCas9 in tomato, we designed 89 g(uide) RNAs targeting genes of the tomato MYB transcription factor family with varying predicted specificities. Plasmids enco...
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| Principais autores: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Frontiers Media S.A.
2023-06-01
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| Serija: | Frontiers in Genome Editing |
| Teme: | |
| Online dostop: | https://www.frontiersin.org/articles/10.3389/fgeed.2023.1196763/full |
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