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A quick, cheap, and reliable protocol for immunofluorescence of pluripotent and differentiating mouse embryonic stem cells in 2D and 3D colonies

Summary: Immunofluorescent labeling is a widely used method to visualize endogenous proteins. It can be expensive and difficult to stain mouse embryonic stem cells (mESCs) because they require expensive growth media, prefer specific substrates, grow in 3D, and have loose cell-substrate adhesion. Her...

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I tiakina i:
Ngā taipitopito rārangi puna kōrero
Ngā kaituhi matua: Snježana Kodba, Agathe Chaigne
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Elsevier 2023-03-01
Rangatū:STAR Protocols
Ngā marau:
Urunga tuihono:http://www.sciencedirect.com/science/article/pii/S2666166722008802
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