An immunofluorescence microscopy assay to discriminate distinct expression patterns of HIV-1 Gag and Nef proteins in HIV-1 provirus-harboring cells.
Over 95% of HIV-1 proviruses are defective and were once considered clinically irrelevant. However, growing evidence shows that these defective proviruses can still be transcribed and translated into viral proteins. Here, we developed an improved immunofluorescence protocol that combines two anti-Ne...
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| Principais autores: | , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Public Library of Science (PLoS)
2026-01-01
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| Serier: | PLoS ONE |
| Online adgang: | https://doi.org/10.1371/journal.pone.0340463 |
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