Ladder-shape melting temperature isothermal amplification of nucleic acids

A novel method, termed ladder-shape melting temperature isothermal amplification (LMTIA), was developed in this study. As a proof of concept, one pair of primers or two pairs of nested primers and a thermostable DNA polymerase were employed to amplify the internal transcribed spacer of Oryza sativa...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής

Κύριοι συγγραφείς:	Deguo Wang, Yongzhen Wang, Meng Zhang, Yongqing Zhang, Juntao Sun, Chunmei Song, Fugang Xiao, Yuan Ping, Chen Pan, Yushan Hu, Chaoqun Wang, Yanhong Liu
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	Taylor & Francis Group 2021-07-01
Σειρά:	BioTechniques
Θέματα:	isothermal amplification ladder-shape melting temperature isothermal amplification (LMTIA) loop-mediated isothermal amplification (LAMP) melting curve nucleic acids detection primer design
Διαθέσιμο Online:	https://www.future-science.com/doi/10.2144/btn-2020-0173
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