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Purification and optimization of conditions for DNA polymerase isolated from thermophile bacteria Bacillus caldolyticus

Тhermophilic bacteria Bacillus caldolyticus isolated from the hot spring in Bansko, Republic of Macedonia, were used for the isolation of DNA polymerase. Bacterial cells were disrupted by sonication and the first step in the purification of DNA polymerase was 40% ammonium sulfate precipitation. This...

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Hauptverfasser: Zoran T Popovski, Tome Nestorovski, Milica Svetozarevic, Elizabeta Miskoska Milevska
Format: Artigo
Sprache:Inglês
Veröffentlicht: Society of Chemists and Technologists of Macedonia 2017-05-01
Schriftenreihe:Macedonian Journal of Chemistry and Chemical Engineering
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Online-Zugang:https://mjcce.org.mk/index.php/MJCCE/article/view/1147
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