Protocol to develop human alveolar macrophage-like cells from mononuclear cells or purified monocytes for use in respiratory biology research

Summary: Human alveolar macrophages are a unique myeloid subset critical for understanding pulmonary diseases and are difficult to access. Here, we present a protocol to generate human alveolar macrophage-like (AML) cells from fresh peripheral blood mononuclear cells or purified monocytes. We descri...

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Detalhes bibliográficos

Principais autores:	Susanta Pahari, Anna-Lena Neehus, Bruce C. Trapnell, Jacinta Bustamante, Jean-Laurent Casanova, Larry S. Schlesinger
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2024-06-01
coleção:	STAR Protocols
Assuntos:	cell biology cell culture cell isolation flow cytometry immunology
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166724002260
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