Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry

Summary: Studying gene function in T cells is crucial for understanding physiology and disease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4+ T cell differentiation in the intestine. We describe steps for isolating naïve CD4+ T cells from mouse spleens and tr...

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Bibliografische Detailangaben

Hauptverfasser:	Jinzhi Duan, Yanan Sun, Juan D. Matute, Richard S. Blumberg
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2023-09-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Flow Cytometry/Mass Cytometry Immunology
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166723004525
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