In vitro Protein Ubiquitination Assays
Ubiquitin can be added to substrate protein as a protein tag by the concerted actions of ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2) and ubiquitin protein ligase (E3). At the present of E1 and ubiquitin, E2 activity can be determined by the thio-ester formation. The E3 activi...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Bio-protocol LLC
2013-10-01
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| Serie: | Bio-Protocol |
| Accesso online: | https://bio-protocol.org/en/bpdetail?id=928&type=0 |
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