Urea supplementation improves mRNA in vitro transcription by decreasing both shorter and longer RNA byproducts

The current letter to the editor describes the presence of RNA byproducts in small-scale in vitro transcription (IVT) reactions as evaluated by capillary gel electrophoresis, asymmetric flow field flow fractionation, immunoblotting, cell-free translation assays, and in IFN reporter cells. We compare...

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Detalhes bibliográficos

Principais autores:	Combes Francis, Pettersson Frida J, Bui Thanh-Huong, Molska Alicja, Komissarov Artem, Parot Jérémie, Borgos Sven Even
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis Group 2024-12-01
Colecção:	RNA Biology
Assuntos:	In vitro transcription urea VSW3 RNA polymerase transcript impurities mRNA byproducts
Acesso em linha:	https://www.tandfonline.com/doi/10.1080/15476286.2024.2321764
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