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Zymographic differentiation of [NiFe]-Hydrogenases 1, 2 and 3 of <it>Escherichia coli</it> K-12

<p>Abstract</p> <p>Background</p> <p>When grown under anaerobic conditions, <it>Escherichia coli</it> K-12 is able to synthesize three active [NiFe]-hydrogenases (Hyd1-3). Two of these hydrogenases are respiratory enzymes catalysing hydrogen oxidation, whereby Hyd-1 is oxygen-tolerant and Hyd-2 is c...

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Kaydedildi:
Asıl Yazarlar: Pinske Constanze, Jaroschinsky Monique, Sargent Frank, Sawers Gary
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: BMC 2012-07-01
Seri Bilgileri:BMC Microbiology
Konular:
NiFe
Hydrogenase
Formate hydrogenlyase
Formate dehydrogenase
Non-denaturating polyacrylamide gel electrophoresis
In-gel activity staining
Redox-dyes
Online Erişim:http://www.biomedcentral.com/1471-2180/12/134
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