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Analysis of Assembly of Synthetic Antibody Fragments: Expression of Functional scFv with Predefined Specificity

In this work, we have investigated parameters important for the assembly of synthetic genes encoding antibody fragments. These genes are constructed from a set of overlapping single-stranded oligonucleotides (primers), which are assembled into a gene sequence in a one-step process using PCR. Using t...

Бүрэн тодорхойлолт

-д хадгалсан:
Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид: Norihiro Kobayashi, Eskil Söderlind, Carl A.K. Borrebaeck
Формат: Artigo
Хэл сонгох:Inglês
Хэвлэсэн: Taylor & Francis Group 1997-09-01
Цуврал:BioTechniques
Онлайн хандалт:https://www.future-science.com/doi/10.2144/97233st09
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