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Rapid Competitive PCR Using Melting Curve Analysis for DNA Quantification

A rapid competitive PCR method was developed to quantify DNA on the LightCycler®. It rests on the quantitative information contained in the melting curves obtained after amplification in the presence of SYBR® Green I. Specific hybridization probes are not required. Heterologous internal standards sh...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Hauptverfasser: Samiya Al-Robaiy, Stefan Rupf, Klaus Eschrich
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis Group 2001-12-01
Schriftenreihe:BioTechniques
Online-Zugang:https://www.future-science.com/doi/10.2144/01316md06
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