Rapid Competitive PCR Using Melting Curve Analysis for DNA Quantification
A rapid competitive PCR method was developed to quantify DNA on the LightCycler®. It rests on the quantitative information contained in the melting curves obtained after amplification in the presence of SYBR® Green I. Specific hybridization probes are not required. Heterologous internal standards sh...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Taylor & Francis Group
2001-12-01
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| Schriftenreihe: | BioTechniques |
| Online-Zugang: | https://www.future-science.com/doi/10.2144/01316md06 |
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