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Fluorescence Recovery after Photobleaching (FRAP) Assay to Measure the Dynamics of Fluorescence Tagged Proteins in Endoplasmic Reticulum Membranes of Plant Cells

In this protocol, we used fluorescence recovery after photobleaching (FRAP) to measure the influence that some mutations and drug treatment have on mobility of a green fluorescent protein (GFP)-fused viral transmembrane protein into endoplasmic reticulum membranes (Serra-Soriano et al., 2014). The p...

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Bibliografische gegevens
Hoofdauteurs: José Navarro, Marta Serra-Soriano, Vicente Pallás
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Bio-protocol LLC 2014-10-01
Reeks:Bio-Protocol
Online toegang:https://bio-protocol.org/en/bpdetail?id=1268&type=0
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