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Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling

Summary: Tandem mass tags data-dependent acquisition (TMT-DDA) as well as data-independent acquisition-based label-free quantification (LFQ-DIA) have become the leading workflows to achieve deep proteome and phosphoproteome profiles. We present a modular pipeline for TMT-DDA and LFQ-DIA that integra...

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Autori principali: Claire Koenig, Ana Martinez-Val, Previn Naicker, Stoyan Stoychev, Justin Jordaan, Jesper V. Olsen
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2023-09-01
Serie:STAR Protocols
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Accesso online:http://www.sciencedirect.com/science/article/pii/S2666166723005038
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