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Toward precision detection of pyrazinamide resistance: critical concentration assessment and rapid molecular method validation

This study evaluated broth microdilution (BMD) and fluorescence PCR melting curve analysis (MeltPro MTB/PZA, targeting pncA mutations) for detecting pyrazinamide (PZA) resistance-associated mutations in rifampicin-resistant tuberculosis (RR-TB). PZA susceptibility was assessed in RR-TB isolates from...

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Principais autores: Yanfeng Zhao, Lili Tian, Nenhan Wang, Hao Chen, Shuangshuang Chen, Lan Yu, Mengdi Pang, Beichuan Ding, Jie Li, Chuanyou Li, Xiaowei Dai
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2026-05-01
Serier:Frontiers in Microbiology
Fag:
critical concentration
MeltPro MTB/PZA assay
minimum inhibitory concentration
PncA gene mutations
pyrazinamide
rifampicin-resistant tuberculosis
Online adgang:https://www.frontiersin.org/articles/10.3389/fmicb.2026.1828630/full
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