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Platform for the rapid construction and evaluation of GPCRs for crystallography in <it>Saccharomyces cerevisiae</it>

<p>Abstract</p> <p>Background</p> <p>Recent successes in the determination of G-protein coupled receptor (GPCR) structures have relied on the ability of receptor variants to overcome difficulties in expression and purification. Therefore, the quick screening of functionally expressed stable receptor...

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Principais autores: Shiroishi Mitsunori, Tsujimoto Hirokazu, Makyio Hisayoshi, Asada Hidetsugu, Yurugi-Kobayashi Takami, Shimamura Tatsuro, Murata Takeshi, Nomura Norimichi, Haga Tatsuya, Iwata So, Kobayashi Takuya
Formato: Artigo
Idioma:Inglês
Publicado: BMC 2012-06-01
Series:Microbial Cell Factories
Assuntos:
G-protein coupled receptor
Membrane protein
High expression
Screening
Receptor variants
Structural study
<it>Saccharomyces cerevisiae</it>
Acceso en liña:http://www.microbialcellfactories.com/content/11/1/78
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