Optimization of highly efficient exogenous-DNA-free Cas9-ribonucleoprotein mediated gene editing in disease susceptibility loci in wheat (Triticum aestivum L.)

The advancement of precision engineering for crop trait improvement is important in the face of rapid population growth, climate change, and disease. To this end, targeted double-stranded break technology using RNA-guided Cas9 has been adopted widely for genome editing in plants. Agrobacterium or pa...

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Principais autores:	Snigdha Poddar, Jaclyn Tanaka, Katherine L. D. Running, Gayan K. Kariyawasam, Justin D. Faris, Timothy L. Friesen, Myeong-Je Cho, Jamie H. D. Cate, Brian Staskawicz
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2023-01-01
coleção:	Frontiers in Plant Science
Assuntos:	wheat gene editing (CRISPR-Cas9) ribonucleoprotein (RNP) DNA-free transgene-free temperature treatment
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fpls.2022.1084700/full
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