A generic HTS assay for kinase screening: Validation for the isolation of an engineered malate kinase.
An end-point ADP/NAD+ acid/alkali assay procedure, directly applicable to library screening of any type of ATP-utilising/ADP producing enzyme activity, was implemented. Typically, ADP production is coupled to NAD+ co-enzyme formation by the conventional addition of pyruvate kinase and lactate dehydr...
Furkejuvvon:
| Váldodahkkit: | , , , , , , , , |
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| Materiálatiipa: | Artigo |
| Giella: | Inglês |
| Almmustuhtton: |
Public Library of Science (PLoS)
2018-01-01
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| Ráidu: | PLoS ONE |
| Liŋkkat: | http://europepmc.org/articles/PMC5819781?pdf=render |
| Fáddágilkorat: |
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