Precise isolation of high-quality RNA from leaves and storage roots of cassava (Manihot esculenta Crantz) for gene expression studies

Abstract Background The isolation of Ribonucleic Acid (RNA) from leaves and storage roots of cassava (Manihot esculenta Crantz) is a challenging one, due to the presence of large amounts of polyphenolic compounds, polysaccharides, and tuber proteins. RNA with high quality and intact integrity is vit...

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Autori principali:	Jane Ijeoma Reuben-Kalu, Kokiladevi Eswaran, Raveendran Muthurajan, Uma Doraiswamy, Balasubramani Venkatasamy, Kavitha Periannagounder Shanmugam
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	SpringerOpen 2023-06-01
Serie:	Bulletin of the National Research Centre
Soggetti:	Cassava (Manihot esculenta Crantz) Leaf and storage root RNA isolation Polyphenols Polysaccharides RT-qPCR
Accesso online:	https://doi.org/10.1186/s42269-023-01058-1
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