Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

We evaluated the ability of a PCR assay to identify Mycobacterium tuberculosis complex (MTBC) from positive BACTEC® 12B broth cultures. A total of 107 sputum samples were processed and inoculated into Ogawa slants and BACTEC® 12B vials. At a growth index (GI) > 30, 1.0 ml of the 12B broth was remove...

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Autors principals:	Ana Cláudia Carvalho Gouveia, Kathleen D. Eisenach, Solange Alves Vinhas, Fabiola Karla Corrêa Ribeiro, Renata Lyrio Peres, Reynaldo Dietze, David Jamil Hadad, Moisés Palaci
Format:	Artigo
Idioma:	Inglês
Publicat:	Fundação Oswaldo Cruz (FIOCRUZ) 2008-06-01
Col·lecció:	Memorias do Instituto Oswaldo Cruz
Matèries:	Mycobacterium tuberculosis identification PCR BACTEC® 12B culture diagnosis
Accés en línia:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000400012
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