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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation

Abstract Background Genome editing has been considered as powerful tool in agricultural fields. However, genome editing progress in cattle has not been fast as in other mammal species, for some disadvantages including long gestational periods, single pregnancy, and high raising cost. Furthermore, te...

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Principais autores: Gyeong-Min Gim, Kyeong-Hyeon Eom, Dong-Hyeok Kwon, Dae-Jin Jung, Dae-Hyun Kim, Jun-Koo Yi, Jae-Jung Ha, Ji-Hyun Lee, Seong-Beom Lee, Woo-Jae Son, Soo-Young Yum, Won-Wu Lee, Goo Jang
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2023-08-01
coleção:Journal of Animal Science and Biotechnology
Assuntos:
Beta-lactoglobulin, Cattle
CRISPR-Cas9
Electroporation
Knockout
MSTN
PRNP
Acesso em linha:https://doi.org/10.1186/s40104-023-00902-8
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