Heterologous Expression of Gene Encoded Thermostable Lipase and Lipolytic Activity
Three lipase genes namely LK2, LK3 and LK5 were successfully sub-cloned into expression vector pET30a. The recombinant vectors were heterologically expressed into Escherichia coli BL21 (DE3) by 1 mM IPTG induced at 37 °C. The protein with the size of 32, 31 and 28 kDa with correspond to LK2, LK3 a...
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| Principais autores: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Journal of Pure and Applied Microbiology
2017-03-01
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| Series: | Journal of Pure and Applied Microbiology |
| Assuntos: | |
| Acceso en liña: | https://microbiologyjournal.org/heterologous-expression-of-gene-encoded-thermostable-lipase-and-lipolytic-activity/ |
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