Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents

Abstract Background Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. Moreover, the ap...

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Autores principales:	Young-Hoon Jeong, Ukjin Kim, Seul-Gi Lee, Bokyeong Ryu, Jin Kim, Artyuhov Igor, Jong Soo Kim, Cho-Rok Jung, Jae-Hak Park, C-Yoon Kim
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	BMC 2020-08-01
Colección:	BMC Biotechnology
Materias:	Vitrification Cryoprotective agent In vitro MSC Spheroid
Acceso en línea:	http://link.springer.com/article/10.1186/s12896-020-00636-9
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