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Gene doctoring: a method for recombineering in laboratory and pathogenic <it>Escherichia coli </it>strains

<p>Abstract</p> <p>Background</p> <p>Homologous recombination mediated by the λ-Red genes is a common method for making chromosomal modifications in <it>Escherichia coli</it>. Several protocols have been developed that differ in the mechanisms by which DNA, carrying regions homologous to the chromos...

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Principais autores: Penn Charles W, Pallen Mark J, Heurlier Karin, Bingle Lewis EH, Lee David J, Busby Stephen JW, Hobman Jon L
Formato: Artigo
Idioma:Inglês
Publicado: BMC 2009-12-01
Series:BMC Microbiology
Acceso en liña:http://www.biomedcentral.com/1471-2180/9/252
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