Optimization of Erythrocyte Lysis Protocols for Robust and Reproducible T Cell Analysis in Murine Peripheral Blood, Spleen, Lung, and Bone Marrow

Flow cytometry analysis of T lymphocytes in murine peripheral blood, spleen, lung, and bone marrow is critical for immune studies. However, inconsistent erythrocyte lysis remains a major challenge—incomplete lysis obscures lymphocyte detection, while excessive lysis damages T cells. Moreover, the va...

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Detalhes bibliográficos

Principais autores:	Wei-Qian Bao, Meng-Lin Jiang, Nan-Jie Zhou, Li-Rong Lian, Shu-qin Li, Bi-qing Huang, Tu-Liang Liang, Gang-Yuan Ma, Cui-Fen Zhang, Ying Chen, Xiao Shu, Liang Liu, Hu-Dan Pan, Run-Ze Li
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Wiley 2026-01-01
Colecção:	Journal of Immunology Research
Acesso em linha:	http://dx.doi.org/10.1155/jimr/3335332
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