Evaluation of Reference Genes to Analyze Gene Expression in Silverside Odontesthes humensis Under Different Environmental Conditions

Some mammalian reference genes, which are widely used to normalize the qRT-PCR, could not be used for this purpose due to its high expression variation. The normalization with false reference genes leads to misinterpretation of results. The silversides (Odontesthes spp.) has been used as models for...

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Главные авторы:	Tony L. R. Silveira, William B. Domingues, Mariana H. Remião, Lucas Santos, Bruna Barreto, Ingrid M. Lessa, Antonio Sergio Varela Junior, Diego Martins Pires, Carine Corcini, Tiago Collares, Fabiana K. Seixas, Ricardo B. Robaldo, Vinicius F. Campos
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Frontiers Media S.A. 2018-03-01
Серии:	Frontiers in Genetics
Предметы:	algorithm expression fish gene normalization real time PCR
Online-ссылка:	http://journal.frontiersin.org/article/10.3389/fgene.2018.00075/full
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