Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9
OCT4 is a crucial transcription factor in the pluripotent stem cell gene regulatory network and an essential factor for pluripotent reprogramming. We engineered the previously reported HEL24.3 hiPSC to generate an OCT4 reporter cell line by knocking-in a T2A nuclear EmGFP reporter cassette before th...
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| Principais autores: | , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Elsevier
2017-08-01
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| Serier: | Stem Cell Research |
| Online adgang: | http://www.sciencedirect.com/science/article/pii/S1873506117301307 |
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