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Optimized protocol for whole-mount RNA fluorescent in situ hybridization using oxidation-mediated autofluorescence reduction on mouse embryos

Summary: Tissue autofluorescence poses significant challenges for RNA and protein analysis using fluorescence-based techniques. Here, we present a protocol that combines oxidation-mediated autofluorescence reduction with detergent-based tissue permeabilization for whole-mount RNA-fluorescence in sit...

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Principais autores: Angela Morabito, Jonas Malkmus, Anna Pancho, Aimée Zuniga, Rolf Zeller, Rushikesh Sheth
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2023-12-01
Serier:STAR Protocols
Fag:
Developmental Biology
Microscopy
Gene Expression
Antibody
In situ Hybridization
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166723005701
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