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Intermolecular dark resonance energy transfer (DRET) for high contrast imaging of endogenous mRNAs in 3D biological samples

Abstract In cellulo detection of RNA molecules is a cornerstone for understanding many biological processes, with recent advances heavily centered on fluorescence-based strategies. The fluorescence resonance energy transfer process stands out as an indispensable tool due to the high specificity of t...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Guillaume Barnoin, Fabienne De Graeve, Aurélien Darnas, Sameh Ben Aicha, Baptiste Monterroso, Alain Burger, Florence Besse, Benoît Y. Michel
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Portfolio 2025-12-01
Schriftenreihe:Scientific Reports
Schlagworte:
Fluorogenic probes
RNA imaging
Oskar mRNA
DRET vs FRET
In situ hybridization
Online-Zugang:https://doi.org/10.1038/s41598-025-27000-1
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