An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation

The plasmid vector pGreenII is widely used to produce plant transformants via a process that involves propagation in Escherichia coli. However, we show here that pGreenII-based constructs can be unstable in E. coli as a consequence of them hampering cell division and promoting cell death. In additio...

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Principais autores:	Michael R. Watson, Yu-fei Lin, Elizabeth Hollwey, Rachel E. Dodds, Peter Meyer, Kenneth J. McDowall
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2016-07-01
coleção:	G3: Genes, Genomes, Genetics
Assuntos:	Agrobacterium plant transformation pGreen E. coli growth defects
Acesso em linha:	http://g3journal.org/lookup/doi/10.1534/g3.116.029405
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