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Effects of DNA extraction methods on the real time PCR quantification of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in chicken feces and ceca contents

ABSTRACT: Polymerase chain reaction (PCR) method was coupled with a DNA extraction to enumerate Campylobacter spp. from poultry gastrointestinal tract samples. Three experiments were conducted that included: 1) Development of a DNA standard curve related to bacterial DNA primers; 2) Design of a cell...

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Bibliografiske detaljer
Principais autores: Paul Dawson, Ahmet Buyukyavuz, Claudia Ionita, Julie Northcutt
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2023-02-01
Serier:Poultry Science
Fag:
Online adgang:http://www.sciencedirect.com/science/article/pii/S0032579122006630
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