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Generation and isolation of single cells from mouse brain with mosaic analysis with double markers-induced uniparental chromosome disomy

Summary: Mosaic analysis with double markers (MADM) technology enables concomitant fluorescent cell labeling and induction of uniparental chromosome disomy (UPD) with single-cell resolution. In UPD, imprinted genes are either overexpressed 2-fold or are not expressed. Here, the MADM platform is util...

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Principais autores: Susanne Laukoter, Nicole Amberg, Florian M. Pauler, Simon Hippenmeyer
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020-12-01
Serier:STAR Protocols
Fag:
Single Cell
Flow Cytometry/Mass Cytometry
Genetics
Neuroscience
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166720302021
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