Cloning of coat protein gene of Grapevine fanleaf virus Hangzhou isolate and construction of its plant expression vector
The coat protein (CP) gene cDNA of Grapevine fanleaf virus (GFLV) Hangzhou isolate was amplified from total viral RNA by RT-PCR, then inserted into pGEM-T-easy Vector. Sequence analysis showed that this gene contained 1515 nucleotide acids encoding 504 amino acids. The nucleotide sequence and deduce...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Zhejiang University Press
2002-11-01
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| Серии: | 浙江大学学报. 农业与生命科学版 |
| Предметы: | |
| Online-ссылка: | https://www.academax.com/doi/10.3785/1008-9209.2002.06.0646 |
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