Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of septic patients

Objective: To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC). Methods: Total RNA in PBMCs was extracted and RT-qPCR was used to determine the mRNA expression profiles of 9 can...

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Detalhes bibliográficos

Principais autores:	Ruoyu Song, Shijun He, Yongbin Wu, Wanxin Chen, Jie Song, Yaxi Zhu, Huan Chen, Qianlu Wang, Shouman Wang, Sichuang Tan, Sipin Tan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2023-04-01
Colecção:	Heliyon
Assuntos:	Sepsis RT-qPCR Reference gene
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2405844023024763
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