Integration-specific In Vitro Evaluation of Lentivirally Transduced Rhesus CD34+ Cells Correlates With In Vivo Vector Copy Number

Hematopoietic stem cell (HSC) gene therapy using integrating vectors has a potential leukemogenic risk due to insertional mutagenesis. To reduce this risk, a limitation of ≤2 average vector copy number (VCN) per cell is generally accepted. We developed an assay for VCN among transduced CD34+ cells t...

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Autores principales:	Naoya Uchida, Molly E Evans, Matthew M Hsieh, Aylin C Bonifacino, Allen E Krouse, Mark E Metzger, Stephanie E Sellers, Cynthia E Dunbar, Robert E Donahue, John F Tisdale
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Elsevier 2013-01-01
Colección:	Molecular Therapy: Nucleic Acids
Materias:	CD34+ cells hematopoietic stem cell transplantation large animal model lentiviral vector
Acceso en línea:	http://www.sciencedirect.com/science/article/pii/S2162253116301810
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