Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier

Abstract A two-stage method of obtaining viable human amniotic stem cells (hAMSCs) in large-scale is described. First, human amniotic stem cells are isolated via dual enzyme (collagenase II and DNAase I) digestion. Next, relying on a culture of the cells from porous chitosan-based microspheres in vi...

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Principais autores:	Bi Foua Claude Alain Gohi, Xue-Ying Liu, Hong-Yan Zeng, Sheng Xu, Kouassi Marius Honore Ake, Xiao-Ju Cao, Kai-Min Zou, Sheila Namulondo
Formato:	Artigo
Idioma:	Inglês
Publicado:	BMC 2020-01-01
Series:	Cell & Bioscience
Assuntos:	hAMSCs Isolation Dual enzyme digestion Expansion Chitosan-based microspheres Enhanced efficiency
Acceso en liña:	https://doi.org/10.1186/s13578-019-0367-y
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