An assay of human tyrosine protein kinase ABL activity using an Escherichia coli protein expression system
ABL, a human tyrosine protein kinase, and its substrate are co-expressed in Escherichia coli. Tyrosine phosphorylation of the substrate in E. coli was detected using Phos-tag SDS-PAGE. The bacterial co-expression system was used as a field for the kinase reaction to evaluate the enzymatic activity o...
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
2021-04-01
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| coleção: | BioTechniques |
| Assuntos: | |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/btn-2020-0154 |
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