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Generation of UL128-shRNA transduced fibroblasts for the release of cell-free virus from clinical human cytomegalovirus isolates

Working with recent isolates of human cytomegalovirus (HCMV) is complicated by their strictly cell-associated growth with lack of infectivity in the supernatant. Adaptation to cell-free growth is associated with disruption of the viral UL128 gene locus. The authors transduced fibroblasts with a lent...

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I tiakina i:
Ngā taipitopito rārangi puna kōrero
Ngā kaituhi matua: Nina Weiler, Kerstin Laib Sampaio, Myriam Scherer, Christian Sinzger
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Taylor & Francis Group 2023-11-01
Rangatū:BioTechniques
Ngā marau:
Urunga tuihono:https://www.future-science.com/doi/10.2144/btn-2023-0046
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