Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction

A comparison of histochemical detection of GM1 ganglioside in cryostat sections using cholera toxin B-subunit after fixation with 4% formaldehyde and dry acetone gave tissue-dependent results. In the liver no pre-treatment showed detectable differences related to GM1 reaction products, while studies...

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Autori principali:	T. Petr, V. Šmíd, J. Šmídová, H. Hůlková, M. Jirkovská, M. Elleder, L. Muchová, L. Vitek, F. Šmíd
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	PAGEPress Publications 2010-05-01
Serie:	European Journal of Histochemistry
Soggetti:	fixation, GM1 ganglioside, cholera toxin, anhydrous acetone, 4% formaldehyde
Accesso online:	http://www.ejh.it/index.php/ejh/article/view/1493
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