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Dissecting efficiency of a 5' rapid amplification of cDNA ends (5'-RACE) approach for profiling T-cell receptor beta repertoire.

Deep sequencing of T-cell receptor (TCR) genes is powerful at profiling immune repertoire. To prepare a TCR sequencing library, multiplex polymerase chain reaction (mPCR) is widely applied and is highly efficient. That is, most mPCR products contain the region critical for antigen recognition, which...

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Príomhchruthaitheoirí: Yu-Hung Lin, Sheng-Jou Hung, Yi-Lin Chen, Cheng-Han Lin, Te-Fang Kung, Yi-Chun Yeh, Joseph T Tseng, Tsunglin Liu
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Public Library of Science (PLoS) 2020-01-01
Sraith:PLoS ONE
Rochtain ar líne:https://doi.org/10.1371/journal.pone.0236366
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