When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping

Abstract Background Genome editing in mice using either classical approaches like homologous recombination or CRISPR/Cas9 has been reported to harbor off target effects (insertion/deletion, frame shifts or gene segment duplications) that lead to mutations not only in close proximity to the target si...

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Bibliografiska uppgifter

Huvudupphov:	Sabrina Sailer, Stefan Coassin, Katharina Lackner, Caroline Fischer, Eileen McNeill, Gertraud Streiter, Christian Kremser, Manuel Maglione, Catherine M. Green, Daniela Moralli, Alexander R. Moschen, Markus A. Keller, Georg Golderer, Gabriele Werner-Felmayer, Irmgard Tegeder, Keith M. Channon, Benjamin Davies, Ernst R. Werner, Katrin Watschinger
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	BMC 2021-03-01
Serie:	Cell & Bioscience
Ämnen:	Homologous recombination Mouse models Ether lipid metabolism Genomic structural variation Alkylglycerol monooxygenase
Länkar:	https://doi.org/10.1186/s13578-021-00566-9
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