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CRISPR/Cas9-based depletion of 16S ribosomal RNA improves library complexity of single-cell RNA-sequencing in planarians

Abstract Background Single-cell RNA-sequencing (scRNA-seq) relies on PCR amplification to retrieve information from vanishingly small amounts of starting material. To selectively enrich mRNA from abundant non-polyadenylated transcripts, poly(A) selection is a key step during library preparation. How...

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Principais autores: Kuang-Tse Wang, Carolyn E. Adler
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2023-10-01
Serier:BMC Genomics
Fag:
scRNA-seq
Ribodepletion
CRISPR/Cas9
DASH
Planarians
Online adgang:https://doi.org/10.1186/s12864-023-09724-4
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