Interlaboratory assays from the fungal PCR Initiative and the Modimucor Study Group to improve qPCR detection of Mucorales DNA in serum: one more step toward standardization

ABSTRACT The aim of this study was to identify parameters influencing DNA extraction and PCR amplification efficiencies in an attempt to standardize Mucorales qPCR. The Fungal PCR Initiative Mucorales Laboratory Working Group distributed two panels of simulated samples to 26 laboratories: Panel A (s...

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Glavni autori:	Steffi Rocchi, Emeline Scherer, P. Lewis White, Audrey Guitton, Alexandre Alanio, Françoise Botterel, Marie Elisabeth Bougnoux, Maria José Buitrago, Massimo Cogliati, Marjorie Cornu, Celine Damiani, Julie Denis, Damien Dupont, Stefan Fuchs, Rebecca Gorton, Pieter-Jan Haas, Ferry Hagen, Rasmus Hare, Xavier Iriart, Corné H. W. Klaassen, Michaela Lackner, Martina Lengerova, Willem J. G. Melchers, Florent Morio, Philippe Poirier, Jan Springer, Stephane Valot, Birgit Willinger, Cristina Mazzi, Mario Cruciani, Rosemary Barnes, J. Peter Donnelly, Jürgen Loeffler, Laurence Millon
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology 2025-02-01
Serija:	Journal of Clinical Microbiology
Teme:	mucormycosis Mucorales PCR standardization interlaboratory assay
Online pristup:	https://journals.asm.org/doi/10.1128/jcm.01525-24
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