An electrophoretic mobility shift assay using the protein isolated from host plants

Abstract Background The electrophoretic mobility shift assay (EMSA) is a common technology to detect DNA-protein interactions. However, in most cases, the protein used in EMSA is obtained via prokaryotic expression, and rarely from plants. At the same time, the proteins expressed from prokaryotic sy...

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Autors principals:	Zihang He, Zhibo Wang, Zhangguo Lu, Caiqiu Gao, Yucheng Wang
Format:	Artigo
Idioma:	Inglês
Publicat:	BMC 2024-05-01
Col·lecció:	Plant Methods
Matèries:	EMSA Fluorescence Cyanine 3 DNA-protein interaction
Accés en línia:	https://doi.org/10.1186/s13007-024-01201-7
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