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High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing.

We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High-Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index...

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Principais autores: Hideo Matsumura, Kentaro Yoshida, Shujun Luo, Eiji Kimura, Takahiro Fujibe, Zayed Albertyn, Roberto A Barrero, Detlev H Krüger, Günter Kahl, Gary P Schroth, Ryohei Terauchi
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science (PLoS) 2010-08-01
Serier:PLoS ONE
Online adgang:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20700453/pdf/?tool=EBI
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