Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Abstract Background Crocus sativus is a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties in Agrobacterium mediated transformation and vegetative reproduction. Effective genome editing requires proficient callus production and an efficient method to d...

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Detalhes bibliográficos

Principais autores:	Sudha Chib, Arulprakash Thangaraj, Sanjana Kaul, Manoj Kumar Dhar, Tanushri Kaul
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2020-04-01
coleção:	Plant Methods
Assuntos:	Cas9 CRISPR Gene manipulation Saffron Invitro regeneration Transformation
Acesso em linha:	http://link.springer.com/article/10.1186/s13007-020-00589-2
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